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Alpha Diagnostics
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Image Search Results
Journal: Diabetes
Article Title: Inhibitory Effects of Leptin on Pancreatic α-Cell Function
doi: 10.2337/db08-1787
Figure Lengend Snippet: Expression of leptin receptors in the pancreatic α-cell. A : PCR analysis of ObR transcripts shows that multiple isoforms are expressed in αTC1-9 cells. The expression in the mouse hypothalamus (Hyp) and islets is also illustrated. B : The presence of ObRb was demonstrated in αTC1-9 cells and in mouse islets by Western blot as well. An antibody that recognizes the extracellular domain of all the isoforms was also tested (ObR). Two examples are shown for the αTC1-9 cells. C and D : The spatial localization of ObRb in αTC1-9 cells and in α-cells within mouse and human islets (green) was assayed by immunofluorecence and confocal microscopy. Glucagon staining is shown in red. As in B , we also used an antibody that recognizes all the isoforms (ObR). These results are representative of at least three different experiments for each condition. Scale bar: 20 μm. (A high-quality representation of this figure is available in the online issue.)
Article Snippet: ObR receptors were detected with an anti-ObR goat antibody (1:100; Santa Cruz Biotechnology, Santa Cruz, CA) or an
Techniques: Expressing, Western Blot, Confocal Microscopy, Staining